The Use of Patient-Derived Explant Cultures for Predicting Breast Cancer Cell Migration Potential In Vitro

Miftahul Husna, Dimas Ramadhian Noor, Rizki Sekar Arum, Hana Qanita, Abinawanto, Anom Bowolaksono, Erwin Danil Julian, Astari Dwiranti


Breast cancer ranks as one of the leading causes of death globally, while mortality rates due to breast cancer continue to rise. In Indonesia, breast cancer is the most common type of cancer, with women dying from breast cancer at a higher rate than men. Primary cultures play a significant role in examining the behavior of breast cancer cells. Because explant cultures can be performed directly from the primary tumor, they constitute a promising new tool for observing cell migration in cancer, including breast cancer. Nevertheless, the use of explant cultures to predict the migration of breast cancer cells has yet to be investigated. This study aims to assess the potential of the explant culture method to predict the migration ability of breast cancer cells in vitro. Tumor explants from two different patients were evaluated in this study. The explant cultures were observed for 14 days until passage, and the results were examined using a microscope. We found that BC02 cells took less than seven days to migrate from the primary tumor, while BC01 cells took 21 days. Furthermore, a mammosphere was observed in the BC02 sample. The rate of cell migration from the tissue depends on the malignant status of the tissue. In conclusion, this study suggests that explant cultures can be used to study the characteristics of cancer cell migration and its correlation with the malignancy of the original tissue.


Keywords: explant culture, cell migration, breast cancer, in vitro.

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